2BMF
Dengue virus RNA helicase at 2.4A
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID14-4 |
| Synchrotron site | ESRF |
| Beamline | ID14-4 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2004-11-04 |
| Detector | ADSC CCD |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 54.900, 178.100, 55.400 |
| Unit cell angles | 90.00, 101.30, 90.00 |
Refinement procedure
| Resolution | 20.000 - 2.410 |
| R-factor | 0.2217 |
| Rwork | 0.222 |
| R-free | 0.27030 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2bhr |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.284 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | AMoRE |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 29.000 | 2.510 |
| High resolution limit [Å] | 2.410 | 2.410 |
| Rmerge | 0.060 | 0.210 |
| Number of reflections | 37606 | |
| <I/σ(I)> | 20.18 | 8.82 |
| Completeness [%] | 92.5 | 82.5 |
| Redundancy | 7 | 6.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 6.5 | pH 6.50 |
