2BM9
cmcI-N160 in complex with SAM
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID14-3 |
| Synchrotron site | ESRF |
| Beamline | ID14-3 |
| Temperature [K] | 100 |
| Collection date | 2004-04-21 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 92.805, 103.027, 182.024 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 55.050 - 2.940 |
| R-factor | 0.267 |
| Rwork | 0.264 |
| R-free | 0.32900 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2bm8 |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.307 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.1.24) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 91.300 | 3.000 |
| High resolution limit [Å] | 2.900 | 2.900 |
| Rmerge | 0.090 | 0.570 |
| Number of reflections | 37586 | |
| <I/σ(I)> | 14 | 2.2 |
| Completeness [%] | 99.9 | 100 |
| Redundancy | 6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 7.5 | pH 7.50 |
