2BM7
The Structure of MfpA (Rv3361c, P3221 Crystal form). The Pentapeptide Repeat Protein from Mycobacterium tuberculosis Folds as A Right- handed Quadrilateral Beta-helix.
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Source details | RIGAKU RU300 |
Temperature [K] | 93 |
Detector technology | IMAGE PLATE |
Detector | MSC RAXIS IV |
Spacegroup name | P 32 2 1 |
Unit cell lengths | 84.080, 84.080, 147.122 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 30.000 - 2.700 |
R-factor | 0.229 |
Rwork | 0.229 |
R-free | 0.28900 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | PREVIOUS STRUCTURE DETERMINED BY SE-MET MAD |
RMSD bond length | 0.011 |
RMSD bond angle | 1.600 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | AMoRE |
Refinement software | CNS (1.0) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 30.000 | 2.800 |
High resolution limit [Å] | 2.700 | 2.700 |
Rmerge | 0.030 | 0.180 |
Number of reflections | 16076 | |
<I/σ(I)> | 22.5 | 6.3 |
Completeness [%] | 94.0 | 90.5 |
Redundancy | 4.5 | 3.9 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | 4 | PROTEIN (10MG/ML, 10% ETHYLENE GLYCOL, 200 MM AMMONIUM SULFATE) CRYSTALLIZED IN 30 % ETHYLENE GLYCOL, 100 MM CITRATE PHOSPHATE PH 4.0. |