2BM3
Structure of the Type II cohesin from Clostridium thermocellum SdbA
Experimental procedure
| Experimental method | MAD |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID14-2 |
| Synchrotron site | ESRF |
| Beamline | ID14-2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2005-02-12 |
| Detector | ADSC CCD |
| Spacegroup name | P 63 2 2 |
| Unit cell lengths | 79.677, 79.677, 101.745 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 69.010 - 1.800 |
| R-factor | 0.192 |
| Rwork | 0.189 |
| R-free | 0.26000 |
| Structure solution method | COMBINED MULTIPLE WAVELENGTH AND MULTIPLE ISOMORPHOUS REPLACEMENT |
| RMSD bond length | 0.015 |
| RMSD bond angle | 1.529 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | SHARP |
| Refinement software | REFMAC (5.2.0005) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | 1.860 |
| High resolution limit [Å] | 1.800 | 1.800 |
| Rmerge | 0.060 | 0.400 |
| Number of reflections | 18256 | |
| <I/σ(I)> | 73.5 | 15 |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 35.2 | 35.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 2MM CACL2, 5% ISOPROPANOL AND 2M AMONNIUM SULPHATE. CRYO-PROTECTED WITH 30% GLYCEROL |
