2BM0
Ribosomal elongation factor G (EF-G) Fusidic acid resistant mutant T84A
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | MAX II BEAMLINE I711 |
Synchrotron site | MAX II |
Beamline | I711 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2004-04-30 |
Detector | MARRESEARCH |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 78.200, 88.500, 116.900 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 25.000 - 2.400 |
R-factor | 0.21 |
Rwork | 0.210 |
R-free | 0.27400 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1fnm |
RMSD bond length | 0.013 |
RMSD bond angle | 1.520 |
Data reduction software | XDS |
Data scaling software | XDS |
Phasing software | MOLREP |
Refinement software | REFMAC (5.0) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 25.000 | 2.600 |
High resolution limit [Å] | 2.400 | 2.400 |
Rmerge | 0.060 | |
Number of reflections | 27567 | |
<I/σ(I)> | 19.1 | 4.1 |
Completeness [%] | 99.6 | 87.9 |
Redundancy | 7.5 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | 7.3 | 17% PEG 8000 100 MM HEPES 46 MM TRIS-HCL PH 7.3 |