2BLI
L29W Mb deoxy
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | ENRAF-NONIUS FR591 |
| Temperature [K] | 295 |
| Detector technology | AREA DETECTOR |
| Collection date | 2002-11-03 |
| Detector | BRUKER MULTIWIRE |
| Spacegroup name | P 6 |
| Unit cell lengths | 91.750, 91.750, 46.180 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 50.000 - 1.700 |
| R-factor | 0.1875 |
| Rwork | 0.188 |
| R-free | 0.20670 |
| Structure solution method | OTHER |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.007 |
| Data reduction software | SAINT |
| Data scaling software | SAINT |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.780 |
| High resolution limit [Å] | 1.700 | 1.700 |
| Rmerge | 0.050 | 0.260 |
| Number of reflections | 99657 | |
| <I/σ(I)> | 11 | 2.8 |
| Completeness [%] | 85.3 | 73.1 |
| Redundancy | 4.7 | 2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 7.8 | 2.8 M AMMONIUMSULFAT, 25 MM TRIS, 1 MM EDTA, PH 8.0 |
