2BLH
Ligand Migration and Protein Fluctuations in Myoglobin Mutant L29W
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Source details | ENRAF-NONIUS FR591 |
Temperature [K] | 295 |
Detector technology | AREA DETECTOR |
Collection date | 2003-09-19 |
Detector | BRUKER, MULTIWIRE |
Spacegroup name | P 6 |
Unit cell lengths | 92.420, 92.420, 46.790 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 50.000 - 1.770 |
R-factor | 0.1865 |
Rwork | 0.186 |
R-free | 0.20240 |
Structure solution method | OTHER |
RMSD bond length | 0.008 |
RMSD bond angle | 1.031 |
Data reduction software | SAINT |
Data scaling software | SAINT |
Refinement software | CNS (1.1) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 1.860 |
High resolution limit [Å] | 1.770 | 1.770 |
Rmerge | 0.030 | 0.180 |
Number of reflections | 81268 | |
<I/σ(I)> | 18.1 | 4.2 |
Completeness [%] | 89.4 | 51 |
Redundancy | 4.2 | 1.8 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | 7.8 | 2.8 M AMMONIUM SULFATE, 25 MM TRIS, 1 MM EDTA, PH 8.0 |