2BLH
Ligand Migration and Protein Fluctuations in Myoglobin Mutant L29W
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | ENRAF-NONIUS FR591 |
| Temperature [K] | 295 |
| Detector technology | AREA DETECTOR |
| Collection date | 2003-09-19 |
| Detector | BRUKER, MULTIWIRE |
| Spacegroup name | P 6 |
| Unit cell lengths | 92.420, 92.420, 46.790 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 50.000 - 1.770 |
| R-factor | 0.1865 |
| Rwork | 0.186 |
| R-free | 0.20240 |
| Structure solution method | OTHER |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.031 |
| Data reduction software | SAINT |
| Data scaling software | SAINT |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.860 |
| High resolution limit [Å] | 1.770 | 1.770 |
| Rmerge | 0.030 | 0.180 |
| Number of reflections | 81268 | |
| <I/σ(I)> | 18.1 | 4.2 |
| Completeness [%] | 89.4 | 51 |
| Redundancy | 4.2 | 1.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 7.8 | 2.8 M AMMONIUM SULFATE, 25 MM TRIS, 1 MM EDTA, PH 8.0 |
