2BL4
Lactaldehyde:1,2-propanediol oxidoreductase of Escherichia coli
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID13 |
| Synchrotron site | ESRF |
| Beamline | ID13 |
| Temperature [K] | 110 |
| Detector technology | CCD |
| Collection date | 2003-11-30 |
| Detector | MARRESEARCH |
| Spacegroup name | P 31 2 1 |
| Unit cell lengths | 109.422, 109.422, 182.459 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 28.000 - 2.850 |
| R-factor | 0.254 |
| Rwork | 0.251 |
| R-free | 0.30300 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1o2d |
| RMSD bond length | 0.017 |
| RMSD bond angle | 1.788 |
| Data reduction software | MOSFLM |
| Data scaling software | XDS |
| Phasing software | AMoRE |
| Refinement software | REFMAC (5.2.0005) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 28.000 | 2.900 |
| High resolution limit [Å] | 2.850 | 2.850 |
| Rmerge | 0.140 | 0.540 |
| Number of reflections | 24555 | |
| <I/σ(I)> | 8.16 | 2.62 |
| Completeness [%] | 81.4 | 67.2 |
| Redundancy | 3.6 | 3.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 6 | PROTEIN WAS CRYSTALLIZED FROM 0.8M AMMONIUM SULPHATE, 0.1M MES PH6, pH 6.00 |
