2BKW
Yeast alanine:glyoxylate aminotransferase YFL030w
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE BM30A |
| Synchrotron site | ESRF |
| Beamline | BM30A |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Detector | MARRESEARCH |
| Spacegroup name | P 31 2 1 |
| Unit cell lengths | 57.400, 57.400, 184.500 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 28.000 - 2.570 |
| R-factor | 0.1985 |
| Rwork | 0.199 |
| R-free | 0.25830 |
| Structure solution method | SAD |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.331 |
| Data reduction software | XDS |
| Data scaling software | CCP4 |
| Phasing software | CNS |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 28.000 | 2.650 |
| High resolution limit [Å] | 2.570 | 2.570 |
| Rmerge | 0.040 | 0.160 |
| Number of reflections | 11595 | |
| <I/σ(I)> | 25.5 | 4.9 |
| Completeness [%] | 97.5 | 92.2 |
| Redundancy | 5.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 0.2 M AMONIUM SULPHATE, 30% PEG MME 2000, 0.1 M SODIUM ACETATE, PH4.5 |
