2BKR
NEDD8 NEDP1 complex
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ESRF BEAMLINE BM14 |
Synchrotron site | ESRF |
Beamline | BM14 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2004-03-01 |
Detector | MARRESEARCH |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 54.638, 74.226, 79.590 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 54.230 - 1.900 |
R-factor | 0.171 |
Rwork | 0.169 |
R-free | 0.20900 |
Structure solution method | MAD |
RMSD bond length | 0.017 |
RMSD bond angle | 1.569 |
Data reduction software | MOSFLM |
Data scaling software | SCALA |
Phasing software | SOLVE |
Refinement software | REFMAC (5.2.0003) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 54.000 | 1.950 |
High resolution limit [Å] | 1.900 | 1.900 |
Rmerge | 0.080 | 0.450 |
Number of reflections | 25411 | |
<I/σ(I)> | 5.5 | 3 |
Completeness [%] | 97.8 | 83.7 |
Redundancy | 6.9 | 3 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | 4.5 | CRYSTALS WERE GROWN BY SETTING-DROP METHOD BY MIXING THE NEDP1-NEDD8 COMPLEX (20MG/ML) WITH EQUAL VOLUME OF RESERVOIR SOLUTION CONTAINING 20%PEG8000, 200MM NACL, 100MM PHOSPHATE CITRATE PH4.5, pH 4.50 |