2BKP
STRUCTURE ANALYSIS OF UNKNOWN FUNCTION PROTEIN
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SPRING-8 BEAMLINE BL26B1 |
Synchrotron site | SPring-8 |
Beamline | BL26B1 |
Temperature [K] | 100 |
Detector technology | IMAGE PLATE |
Collection date | 2003-10-23 |
Detector | RIGAKU IMAGE PLATE |
Spacegroup name | P 42 21 2 |
Unit cell lengths | 71.052, 71.052, 98.180 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 33.410 - 2.200 |
R-factor | 0.225 |
Rwork | 0.225 |
R-free | 0.29000 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1vct |
RMSD bond length | 0.007 |
RMSD bond angle | 1.300 |
Data reduction software | HKL-2000 |
Data scaling software | SCALEPACK |
Phasing software | CNS |
Refinement software | CNS (1.1) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 35.000 | 2.280 |
High resolution limit [Å] | 2.200 | 2.200 |
Rmerge | 0.090 | 0.430 |
Number of reflections | 13395 | |
<I/σ(I)> | 32.1 | 5.4 |
Completeness [%] | 99.9 | 100 |
Redundancy | 13.4 | 13.5 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | 4.7 | PROTEIN WAS CRYSTALLIZED FROM 1.25 M NACL, 50 MM ACETATE, PH 4.7; THEN SOAKED WITH 1.5 M KCL. |