2BKM
Crystal structure of the truncated hemoglobin from Geobacillus stearothermophilus
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ELETTRA BEAMLINE 5.2R |
| Synchrotron site | ELETTRA |
| Beamline | 5.2R |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Detector | MARRESEARCH |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 35.123, 99.339, 35.252 |
| Unit cell angles | 90.00, 105.59, 90.00 |
Refinement procedure
| Resolution | 25.000 - 1.500 |
| R-factor | 0.168 |
| Rwork | 0.167 |
| R-free | 0.19400 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1ux8 |
| RMSD bond length | 0.015 |
| RMSD bond angle | 1.634 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.2.0005) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 40.000 | 1.550 |
| High resolution limit [Å] | 1.500 | 1.500 |
| Rmerge | 0.020 | 0.060 |
| Number of reflections | 36594 | |
| Completeness [%] | 97.5 | 93.7 |
| Redundancy | 3.6 | 3.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 5 | PEG 5000 MME 20-25 %, ACETATE BUFFER 0.1 M PH 5.2-6.0 |
