2BKI

Myosin VI nucleotide-free (MDinsert2-IQ) crystal structure

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID23-1
Synchrotron site	ESRF
Beamline	ID23-1
Temperature [K]	100
Detector technology	CCD
Detector	ADSC CCD
Spacegroup name	P 21 21 2
Unit cell lengths	104.781, 250.167, 67.274
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	40.000 - 2.900
R-factor	0.266
Rwork	0.264
R-free	0.30400
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1oe9
RMSD bond length	0.013
RMSD bond angle	1.149
Data reduction software	MOSFLM
Data scaling software	SCALA
Phasing software	AMoRE
Refinement software	REFMAC (5.1.24)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	40.000	3.060
High resolution limit [Å]	2.900	2.900
Rmerge	0.120	0.440
Number of reflections	38961
<I/σ(I)>	16.3	3.7
Completeness [%]	97.0	97
Redundancy	6.3

Crystallization Conditions

crystal ID	method	pH	temperature	details
1				8-10% PEG 8000, 50MM MES PH 6.7, 150MM NH4.SO4, 3% ISO-PROPANOL, 3% TERT-BUTANOL