2BKF
Structure of the PB1 domain of NBR1
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | EMBL/DESY, HAMBURG BEAMLINE X13 |
| Synchrotron site | EMBL/DESY, HAMBURG |
| Beamline | X13 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2003-11-04 |
| Detector | MARRESEARCH |
| Spacegroup name | P 63 2 2 |
| Unit cell lengths | 100.612, 100.612, 42.160 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 21.780 - 1.560 |
| R-factor | 0.2 |
| Rwork | 0.199 |
| R-free | 0.22600 |
| Structure solution method | MAD |
| RMSD bond length | 0.014 |
| RMSD bond angle | 1.484 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | SOLVE |
| Refinement software | REFMAC (5.2.0005) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | 1.610 |
| High resolution limit [Å] | 1.560 | 1.550 |
| Rmerge | 0.060 | 0.590 |
| Number of reflections | 18381 | |
| <I/σ(I)> | 46.6 | 4.8 |
| Completeness [%] | 99.6 | 100 |
| Redundancy | 18.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 4.1 | 1.6 M AMMONIUM SULFATE,0.1 M SODIUM ACETATE PH 4.1 |
