2BKE
Conformational Flexibility Revealed by the Crystal Structure of a Crenarchaeal RadA
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID14-4 |
| Synchrotron site | ESRF |
| Beamline | ID14-4 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2003-09-26 |
| Detector | ADSC CCD |
| Spacegroup name | P 31 2 1 |
| Unit cell lengths | 98.146, 98.146, 99.190 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 84.520 - 3.200 |
| R-factor | 0.178 |
| Rwork | 0.174 |
| R-free | 0.24000 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1N0W CHAIN A |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.235 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.2.0003) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 60.000 | 3.310 |
| High resolution limit [Å] | 3.200 | 3.200 |
| Rmerge | 0.120 | 0.320 |
| Number of reflections | 9023 | |
| <I/σ(I)> | 13.1 | 2.1 |
| Completeness [%] | 96.3 | 74.8 |
| Redundancy | 10 | 8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 9.5 | 100MM TRIS PH 9.5 10% (V/V) TERT-BUTANOL |
