2BKC
The X-ray structure of the H43G Listeria innocua Dps mutant
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ELETTRA BEAMLINE 5.2R |
| Synchrotron site | ELETTRA |
| Beamline | 5.2R |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Detector | MARRESEARCH |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 90.726, 175.648, 135.957 |
| Unit cell angles | 90.00, 92.53, 90.00 |
Refinement procedure
| Resolution | 25.000 - 2.300 |
| R-factor | 0.212 |
| Rwork | 0.209 |
| R-free | 0.26600 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1qgh |
| RMSD bond length | 0.018 |
| RMSD bond angle | 1.593 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | AMoRE |
| Refinement software | REFMAC (5.1.24) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.380 |
| High resolution limit [Å] | 2.300 | 2.300 |
| Rmerge | 0.080 | 0.300 |
| Number of reflections | 172339 | |
| <I/σ(I)> | 10 | 5 |
| Completeness [%] | 91.5 | 89.7 |
| Redundancy | 2 | 2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | PEG 100 15-30% W/V, ACETATE BUFFERS AT PH VALUES BETWEEN 5.5-6.5 |
