2BK9

Drosophila Melanogaster globin

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID29
Synchrotron site	ESRF
Beamline	ID29
Temperature [K]	100
Spacegroup name	P 21 21 21
Unit cell lengths	41.752, 55.835, 56.163
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	28.750 - 1.200
R-factor	0.102
Rwork	0.101
R-free	0.13400
Structure solution method	OTHER
Starting model (for MR)	NONE
RMSD bond length	0.019
RMSD bond angle	1.818
Data reduction software	MOSFLM
Data scaling software	SCALA
Refinement software	REFMAC (5.2.0003)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	20.000	1.260
High resolution limit [Å]	1.200	1.200
Rmerge	0.130	0.110
Number of reflections	36706
<I/σ(I)>	12	7
Completeness [%]	87.9	92
Redundancy	4	3

Crystallization Conditions

crystal ID	method	pH	temperature	details
1		10		pH 10