2BJW
PspF AAA domain
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SRS BEAMLINE PX14.1 |
| Synchrotron site | SRS |
| Beamline | PX14.1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2004-07-16 |
| Detector | ADSC CCD |
| Spacegroup name | P 65 |
| Unit cell lengths | 113.695, 113.695, 39.277 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 100.000 - 1.750 |
| R-factor | 0.18041 |
| Rwork | 0.180 |
| R-free | 0.20785 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2bjv |
| RMSD bond length | 0.022 |
| RMSD bond angle | 1.696 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | PHASER |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.810 |
| High resolution limit [Å] | 1.750 | 1.750 |
| Rmerge | 0.050 | 0.450 |
| Number of reflections | 28281 | |
| <I/σ(I)> | 33.7 | 3.2 |
| Completeness [%] | 95.5 | 80.4 |
| Redundancy | 5.4 | 3.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 8 | 2.0 M AMMONIUM FORMATE, 0.1 M HEPES PH 8.0, 10% MPD |
