2BJO
Crystal Structure of the Organic Hydroperoxide Resistance Protein OhrB of Bacillus subtilis
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | ENRAF-NONIUS FR591 |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2003-12-19 |
| Detector | RIGAKU IMAGE PLATE |
| Spacegroup name | P 1 |
| Unit cell lengths | 35.340, 41.434, 44.600 |
| Unit cell angles | 84.45, 91.51, 73.89 |
Refinement procedure
| Resolution | 44.320 - 2.100 |
| R-factor | 0.161 |
| Rwork | 0.158 |
| R-free | 0.19900 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1n2f |
| RMSD bond length | 0.010 |
| RMSD bond angle | 2.430 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | AMoRE |
| Refinement software | REFMAC (5.2.0005) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 2.180 |
| High resolution limit [Å] | 2.100 | 2.100 |
| Rmerge | 0.040 | 0.250 |
| Number of reflections | 12453 | |
| <I/σ(I)> | 22.3 | 3 |
| Completeness [%] | 88.6 | 46.6 |
| Redundancy | 2.3 | 2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 6.5 | 1.0 M SODIUM CITRATE AND 0.1 M CACODYLATE (PH 6.5). |
