2BJE
Acylphosphatase from Sulfolobus solfataricus. Monclinic P21 space group
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ELETTRA BEAMLINE 5.2R |
Synchrotron site | ELETTRA |
Beamline | 5.2R |
Temperature [K] | 100 |
Detector technology | CCD |
Detector | MARRESEARCH |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 48.980, 56.532, 60.805 |
Unit cell angles | 90.00, 103.69, 90.00 |
Refinement procedure
Resolution | 8.000 - 1.900 |
R-factor | 0.166 |
Rwork | 0.162 |
R-free | 0.21500 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 2acy |
RMSD bond length | 0.020 |
RMSD bond angle | 1.997 |
Data reduction software | MOSFLM |
Data scaling software | SCALA |
Phasing software | PHASER |
Refinement software | REFMAC (5.2.0005) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 29.540 | 1.930 |
High resolution limit [Å] | 1.900 | 1.900 |
Rmerge | 0.080 | 0.330 |
Number of reflections | 25338 | |
<I/σ(I)> | 9.7 | 2.9 |
Completeness [%] | 98.3 | 95.7 |
Redundancy | 5.2 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 |