2BJA
Crystal Analysis of 1-Pyrroline-5-Carboxylate Dehydrogenase from Thermus with bound NADH
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SPRING-8 BEAMLINE BL26B1 |
Synchrotron site | SPring-8 |
Beamline | BL26B1 |
Temperature [K] | 100 |
Detector technology | IMAGE PLATE |
Collection date | 2004-02-14 |
Detector | RIGAKU IMAGE PLATE |
Spacegroup name | H 3 |
Unit cell lengths | 102.007, 102.007, 278.319 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 37.290 - 1.900 |
R-factor | 0.176 |
Rwork | 0.176 |
R-free | 0.21200 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1uzb |
RMSD bond length | 0.007 |
RMSD bond angle | 1.400 |
Data reduction software | HKL-2000 |
Data scaling software | SCALEPACK |
Phasing software | CNS |
Refinement software | CNS (1.1) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 40.000 | 1.940 |
High resolution limit [Å] | 1.900 | 1.900 |
Rmerge | 0.080 | 0.460 |
Number of reflections | 85247 | |
<I/σ(I)> | 17.26 | 2.15 |
Completeness [%] | 100.0 | 100 |
Redundancy | 4 | 3 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | 5.2 | PROTEIN WAS CRYSTALLIZED FROM 36% MPD, 50 MM SODIUM CITRATE/HCL, PH 5.2; THEN SOAKED IN 1 MM NAD,CA.5MM DELTA1-PYRROLINE-5 CARBOXYLATE AND 50 MM SODIUM ACETATE/HCL, PH 5.2. |