2BJ7
NIKR IN CLOSED CONFORMATION AND NICKEL BOUND TO HIGH-AFFINITY SITES
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SPRING-8 BEAMLINE BL26B1 |
Synchrotron site | SPring-8 |
Beamline | BL26B1 |
Temperature [K] | 100 |
Detector technology | IMAGE PLATE |
Collection date | 2004-04-13 |
Detector | RIGAKU RAXIS-V |
Spacegroup name | P 32 2 1 |
Unit cell lengths | 109.952, 109.952, 79.003 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 29.450 - 2.100 |
R-factor | 0.196 |
Rwork | 0.196 |
R-free | 0.21500 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 2bj1 |
RMSD bond length | 0.006 |
RMSD bond angle | 1.100 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | CNS |
Refinement software | CNS (1.1) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 30.000 | 2.180 |
High resolution limit [Å] | 2.100 | 2.100 |
Rmerge | 0.060 | 0.280 |
Number of reflections | 32158 | |
<I/σ(I)> | 24.8 | 5.5 |
Completeness [%] | 99.3 | 99.2 |
Redundancy | 8.3 | 5.9 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | 7.5 | pH 7.50 |