2BJ3
NIKR-apo
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SPRING-8 BEAMLINE BL26B1 |
| Synchrotron site | SPring-8 |
| Beamline | BL26B1 |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2004-04-10 |
| Detector | RIGAKU RAXIS-V |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 75.783, 54.322, 77.276 |
| Unit cell angles | 90.00, 116.06, 90.00 |
Refinement procedure
| Resolution | 29.250 - 2.200 |
| R-factor | 0.218 |
| Rwork | 0.218 |
| R-free | 0.26800 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2bj1 |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.200 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | DM |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 2.280 |
| High resolution limit [Å] | 2.200 | 2.200 |
| Rmerge | 0.050 | 0.280 |
| Number of reflections | 28283 | |
| <I/σ(I)> | 21.9 | 4.5 |
| Completeness [%] | 97.8 | 94.7 |
| Redundancy | 3.1 | 2.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 7.5 | pH 7.50 |
