2BJ3
NIKR-apo
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SPRING-8 BEAMLINE BL26B1 |
Synchrotron site | SPring-8 |
Beamline | BL26B1 |
Temperature [K] | 100 |
Detector technology | IMAGE PLATE |
Collection date | 2004-04-10 |
Detector | RIGAKU RAXIS-V |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 75.783, 54.322, 77.276 |
Unit cell angles | 90.00, 116.06, 90.00 |
Refinement procedure
Resolution | 29.250 - 2.200 |
R-factor | 0.218 |
Rwork | 0.218 |
R-free | 0.26800 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 2bj1 |
RMSD bond length | 0.007 |
RMSD bond angle | 1.200 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | DM |
Refinement software | CNS (1.1) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 30.000 | 2.280 |
High resolution limit [Å] | 2.200 | 2.200 |
Rmerge | 0.050 | 0.280 |
Number of reflections | 28283 | |
<I/σ(I)> | 21.9 | 4.5 |
Completeness [%] | 97.8 | 94.7 |
Redundancy | 3.1 | 2.5 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | 7.5 | pH 7.50 |