2BIT
Crystal structure of human cyclophilin D at 1.7 A resolution
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | NONIUS |
| Temperature [K] | 120 |
| Detector technology | IMAGE PLATE |
| Collection date | 2001-01-15 |
| Detector | MARRESEARCH |
| Spacegroup name | P 41 21 2 |
| Unit cell lengths | 57.165, 57.165, 87.227 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 15.000 - 1.710 |
| R-factor | 0.141 |
| Rwork | 0.139 |
| R-free | 0.17500 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1swj |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.098 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | AMoRE |
| Refinement software | REFMAC (5.1.24) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 15.000 | 1.820 |
| High resolution limit [Å] | 1.710 | 1.710 |
| Rmerge | 0.050 | 0.140 |
| Number of reflections | 91268 | |
| <I/σ(I)> | 20.8 | 10.6 |
| Completeness [%] | 99.2 | 98.5 |
| Redundancy | 5.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 7 | 31 MG/ML PROTEIN, 50 MM NA-PHOSPHATE (PH 7.3), 100 MM NACL, 2MM EDTA, 30% JEFFAMINE |
