2BIR
ADDITIVITY OF SUBSTRATE BINDING IN RIBONUCLEASE T1 (Y42A MUTANT)
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Temperature [K] | 293 |
Detector technology | IMAGE PLATE |
Collection date | 1995-11-06 |
Detector | MARRESEARCH |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 49.610, 48.490, 40.610 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 10.000 - 2.300 |
R-factor | 0.216 |
Rwork | 0.216 |
R-free | 0.25700 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1bir |
RMSD bond length | 0.032 |
RMSD bond angle | 26.100 * |
Data reduction software | MOSFLM |
Data scaling software | CCP4 ((ROTAVATA)) |
Phasing software | X-PLOR (3.843) |
Refinement software | X-PLOR (3.843) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 10.000 | 2.380 |
High resolution limit [Å] | 2.300 | 2.300 |
Rmerge | 0.107 * | 0.239 * |
Number of reflections | 4313 | |
<I/σ(I)> | 5.5 | 3.1 |
Completeness [%] | 93.0 * | 49.7 * |
Redundancy | 5 | 4.2 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | Vapor diffusion, sitting drop * | 4.2 | pH 4.20 |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | drop | protein | 20 (mg/ml) | |
2 | 1 | drop | sodium acetate | 50 (mM) | |
3 | 1 | reservoir | 2'-GMP | 0.125 (%(w/v)) | |
4 | 1 | reservoir | 1.25 (%(w/v)) | ||
5 | 1 | reservoir | MPD | 50 (%(v/v)) |