2BII
crystal structure of nitrate-reducing fragment of assimilatory nitrate reductase from Pichia angusta
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | MPG/DESY, HAMBURG BEAMLINE BW6 |
Synchrotron site | MPG/DESY, HAMBURG |
Beamline | BW6 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2003-10-23 |
Detector | MARRESEARCH |
Spacegroup name | C 2 2 21 |
Unit cell lengths | 122.722, 123.080, 149.509 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 87.710 - 1.700 |
R-factor | 0.186 |
Rwork | 0.185 |
R-free | 0.21200 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 2bih |
RMSD bond length | 0.018 |
RMSD bond angle | 1.996 |
Data reduction software | MOSFLM |
Data scaling software | SCALA |
Phasing software | AMoRE |
Refinement software | REFMAC (5.2.0005) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 25.000 | 1.790 |
High resolution limit [Å] | 1.700 | 1.700 |
Rmerge | 0.090 | 0.510 |
Number of reflections | 2235039 | |
<I/σ(I)> | 4.6 | 1.6 |
Completeness [%] | 99.0 | 99.8 |
Redundancy | 4.3 | 2.2 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 |