2BHY
Crystal structure of Deinococcus radiodurans maltooligosyltrehalose trehalohydrolase in complex with trehalose
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ESRF BEAMLINE ID14-2 |
Synchrotron site | ESRF |
Beamline | ID14-2 |
Temperature [K] | 100 |
Collection date | 2004-02-05 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 60.029, 66.556, 153.141 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 76.700 - 1.500 |
R-factor | 0.129 |
Rwork | 0.128 |
R-free | 0.15200 |
Structure solution method | OTHER |
RMSD bond length | 0.011 |
RMSD bond angle | 1.462 |
Data reduction software | MOSFLM |
Data scaling software | SCALA |
Refinement software | REFMAC (5.2.0003) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 20.000 | 1.580 |
High resolution limit [Å] | 1.500 | 1.500 |
Rmerge | 0.050 | 0.260 |
Number of reflections | 98045 | |
<I/σ(I)> | 14.8 | 3.9 |
Completeness [%] | 99.1 | 95.8 |
Redundancy | 3.4 | 2.7 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | 8.5 | 20% (W/V) PEG 2000 MME, 25% (W/V) TREHALOSE, 0.1 M TRIS-HCL PH 8.5 AND 0.1 M MGCL2 |