2BHX
Radiation damage of the Schiff base in phosphoserine aminotransferase (structure A)
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ESRF BEAMLINE ID14-4 |
Synchrotron site | ESRF |
Beamline | ID14-4 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2004-05-14 |
Detector | ADSC CCD |
Spacegroup name | P 21 21 2 |
Unit cell lengths | 143.016, 83.761, 66.698 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 15.000 - 1.680 |
R-factor | 0.1796 |
R-free | 0.22790 |
Structure solution method | OTHER |
RMSD bond length | 0.007 |
RMSD bond angle | 0.024 |
Data reduction software | HKL |
Data scaling software | HKL |
Refinement software | SHELXL-97 |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 15.000 | 1.720 |
High resolution limit [Å] | 1.680 | 1.680 |
Rmerge | 0.080 | 0.520 |
Number of reflections | 92486 | |
<I/σ(I)> | 11 | 2 |
Completeness [%] | 98.7 | 99.3 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | 8.2 | 30% PEG 400, 200 MM MGCL2, 5% GLYCEROL, 100 MM TRIS PH 8.2 |