2BHV
Structure of ComB10 of the Com Type IV secretion system of Helicobacter pylori
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID23-1 |
| Synchrotron site | ESRF |
| Beamline | ID23-1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2004-03-01 |
| Detector | ADSC CCD |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 69.735, 139.411, 168.442 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 20.000 - 3.000 |
| R-factor | 0.259 |
| Rwork | 0.257 |
| R-free | 0.29300 |
| Structure solution method | OTHER |
| RMSD bond length | 0.015 |
| RMSD bond angle | 1.274 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | SHARP |
| Refinement software | REFMAC (5.2.0005) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | 3.160 |
| High resolution limit [Å] | 3.000 | 3.000 |
| Rmerge | 0.090 | 0.610 |
| Number of reflections | 32829 | |
| <I/σ(I)> | 11 | 2.3 |
| Completeness [%] | 97.4 | 98 |
| Redundancy | 3.7 | 3.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 6 | 8-14% (W/V) POLYETHYLENE GLYCOL (PEG) 8000, 100MM SODIUM CACODYLATE PH6.0 AND 200 MM MAGNESIUM ACETATE, pH 6.00 |
