2BHO
Crystal structure of the Yersinia enterocolitica type III secretion chaperone SycT
Experimental procedure
| Experimental method | MAD |
| Source type | SYNCHROTRON |
| Source details | MPG/DESY, HAMBURG BEAMLINE BW6 |
| Synchrotron site | MPG/DESY, HAMBURG |
| Beamline | BW6 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2004-03-14 |
| Detector | MARRESEARCH |
| Wavelength(s) | 1.0723, 1.0719, 0.98 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 91.170, 45.834, 34.453 |
| Unit cell angles | 90.00, 105.29, 90.00 |
Refinement procedure
| Resolution | 15.000 - 2.600 |
| R-factor | 0.245 |
| Rwork | 0.244 |
| R-free | 0.25900 |
| Structure solution method | MAD |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.256 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | SHELX |
| Refinement software | REFMAC (5.2.0005) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 99.000 | 2.540 |
| High resolution limit [Å] | 2.500 | 2.510 |
| Rmerge | 0.030 | 0.130 |
| Number of reflections | 99007 | |
| <I/σ(I)> | 36.5 | 6 |
| Completeness [%] | 97.4 | 88.9 |
| Redundancy | 5.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 7 | 1.2 M DL-MALIC ACID, PH 7.0 100MM BIS-TRIS PROPANE, PH 7.0 |
