2BHM

Crystal structure of VirB8 from Brucella suis

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID14-4
Synchrotron site	ESRF
Beamline	ID14-4
Temperature [K]	100
Detector technology	CCD
Detector	ADSC CCD
Spacegroup name	I 41 2 2
Unit cell lengths	202.757, 202.757, 103.089
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	26.600 - 2.400
R-factor	0.2456
Rwork	0.246
R-free	0.27330
Structure solution method	OTHER
RMSD bond length	0.008
RMSD bond angle	1.300
Data reduction software	MOSFLM
Data scaling software	SCALA
Phasing software	SHELXD
Refinement software	CNS (1.1)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	22.600	2.530
High resolution limit [Å]	2.400	2.400
Rmerge	0.100	0.440
Number of reflections	44402
<I/σ(I)>	6.3	2.2
Completeness [%]	98.8	98.8
Redundancy	7.3	7.2

Crystallization Conditions

crystal ID	method	pH	temperature	details
1