2BH0
Crystal structure of a SeMet derivative of EXPA from Bacillus subtilis at 2.5 angstrom
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE BM30A |
| Synchrotron site | ESRF |
| Beamline | BM30A |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2004-11-06 |
| Detector | MARRESEARCH |
| Spacegroup name | I 2 3 |
| Unit cell lengths | 144.035, 144.035, 144.035 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 25.460 - 2.500 |
| R-factor | 0.203 |
| Rwork | 0.203 |
| R-free | 0.23100 |
| Structure solution method | SAD |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.300 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALEPACK |
| Phasing software | SOLVE |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 25.460 | 2.640 |
| High resolution limit [Å] | 2.500 | 2.500 |
| Rmerge | 0.130 | 0.680 |
| Number of reflections | 17348 | |
| <I/σ(I)> | 31.6 | 5.1 |
| Completeness [%] | 99.9 | 99.9 |
| Redundancy | 21.8 | 21.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 4M NAFORMATE |
