2BGW

XPF from Aeropyrum pernix, complex with DNA

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SRS BEAMLINE PX9.6
Synchrotron site	SRS
Beamline	PX9.6
Temperature [K]	120
Detector technology	CCD
Detector	ADSC CCD
Spacegroup name	P 32 2 1
Unit cell lengths	141.312, 141.312, 85.311
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	30.000 - 2.800
R-factor	0.225
Rwork	0.223
R-free	0.26300
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1j25
RMSD bond length	0.015
RMSD bond angle	1.959
Data reduction software	MOSFLM
Data scaling software	SCALA
Phasing software	MOLREP
Refinement software	REFMAC (5.2.0005)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	46.100	2.950
High resolution limit [Å]	2.800	2.800
Rmerge	0.080	0.400
Number of reflections	21619
<I/σ(I)>	8.1	1.9
Completeness [%]	97.5	92.8
Redundancy	5.4	2.8

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP			0.1M SODIUM ACETATE, 0.2M AMMONIUM SULFATE, 20% PEG MME 2K, HANGING DROP, ROOM TEMPERATURE