2BGI
X-Ray Structure of the Ferredoxin-NADP(H) Reductase from Rhodobacter capsulatus complexed with three molecules of the detergent n-heptyl- beta-D-thioglucoside at 1.7 Angstroms
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ESRF BEAMLINE ID14-2 |
Synchrotron site | ESRF |
Beamline | ID14-2 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2004-05-17 |
Detector | ADSC CCD |
Spacegroup name | P 31 2 1 |
Unit cell lengths | 120.848, 120.848, 50.709 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 45.640 - 1.680 |
R-factor | 0.221 |
Rwork | 0.221 |
R-free | 0.22500 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1a8p |
RMSD bond length | 0.007 |
RMSD bond angle | 1.600 |
Data reduction software | MOSFLM |
Data scaling software | SCALA |
Phasing software | AMoRE |
Refinement software | CNS (1.0) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 45.600 | 1.800 |
High resolution limit [Å] | 1.700 | 1.700 |
Rmerge | 0.120 | 0.650 |
Number of reflections | 61984 | |
<I/σ(I)> | 11.2 | 2.6 |
Completeness [%] | 97.2 | 90 |
Redundancy | 7.3 | 7.5 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | 6.5 | pH 6.50 |