2BGG
The structure of a Piwi protein from Archaeoglobus fulgidus complexed with a 16nt siRNA duplex.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID14-1 |
| Synchrotron site | ESRF |
| Beamline | ID14-1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2004-12-01 |
| Detector | ADSC CCD |
| Spacegroup name | P 1 |
| Unit cell lengths | 51.916, 61.206, 104.059 |
| Unit cell angles | 76.54, 76.14, 79.35 |
Refinement procedure
| Resolution | 99.010 - 2.200 |
| R-factor | 0.183 |
| Rwork | 0.180 |
| R-free | 0.24300 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1w9h |
| RMSD bond length | 0.022 |
| RMSD bond angle | 2.097 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.2.0005) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 60.000 | 2.320 |
| High resolution limit [Å] | 2.200 | 2.200 |
| Rmerge | 0.070 | 0.400 |
| Number of reflections | 58841 | |
| <I/σ(I)> | 7.5 | 1.2 |
| Completeness [%] | 96.7 | 96.5 |
| Redundancy | 2 | 2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 |
