2BFW
Structure of the C domain of glycogen synthase from Pyrococcus abyssi
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE BM16 |
| Synchrotron site | ESRF |
| Beamline | BM16 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2004-11-12 |
| Detector | MARRESEAERCH |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 39.614, 50.820, 44.261 |
| Unit cell angles | 90.00, 106.89, 90.00 |
Refinement procedure
| Resolution | 42.330 - 1.800 |
| R-factor | 0.186 |
| Rwork | 0.183 |
| R-free | 0.23500 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1rzu |
| RMSD bond length | 0.008 |
| RMSD bond angle | 0.989 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.2.0005) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 37.900 | 1.900 |
| High resolution limit [Å] | 1.800 | 1.800 |
| Rmerge | 0.050 | 0.250 |
| Number of reflections | 15701 | |
| <I/σ(I)> | 15.2 | 3.5 |
| Completeness [%] | 97.6 | 85.9 |
| Redundancy | 2.4 | 2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 4.6 | 30% PEG8K, 0.1 M NAAC PH 4.5, 0.2M LITHIUM SULPHATE |
