2BFR

The Macro domain is an ADP-ribose binding module

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID29
Synchrotron site	ESRF
Beamline	ID29
Temperature [K]	100
Collection date	2004-05-02
Spacegroup name	P 61
Unit cell lengths	87.840, 87.840, 61.069
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	25.000 - 2.500
R-factor	0.202
Rwork	0.202
R-free	0.23720
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1hjz
RMSD bond length	0.012
RMSD bond angle	0.600
Data reduction software	MOSFLM
Data scaling software	SCALA
Phasing software	MOLREP
Refinement software	CNS (1.1)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	28.750	2.630
High resolution limit [Å]	2.500	2.500
Rmerge	0.060	0.240
Number of reflections	9073
<I/σ(I)>	12.2	4.7
Completeness [%]	96.1	97.1
Redundancy	2.4	2.3

Crystallization Conditions

crystal ID	method	pH	temperature	details
1		4.5		20% PEG 8000, 0.2 MAGNESIUM ACETATE, 0.1M SODIUM CACODYLATE (PH 4.5). 18MG/ML PROTEIN, 1.5MM ADP, 5MM DTT. CRYOBUFFER CONTAINED 20% GLYCEROL.