2BF9
Anisotropic refinement of avian (turkey) pancreatic polypeptide at 0. 99 Angstroms resolution.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SEALED TUBE |
| Source details | HILGER-WATTS 1.2KW |
| Temperature [K] | 293 |
| Detector technology | Y290 SCINTILLATION COUNTER |
| Collection date | 1983-01-01 |
| Detector | HILGER-WATTS |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 34.180, 32.920, 28.450 |
| Unit cell angles | 90.00, 105.26, 90.00 |
Refinement procedure
| Resolution | 27.450 - 0.990 |
| R-factor | 0.1368 |
| R-free | 0.17940 |
| Structure solution method | SIRAS |
| RMSD bond length | 0.013 |
| RMSD bond angle | 0.026 |
| Data reduction software | ON-LINE (CENTROID INTEGRATION) |
| Data scaling software | HRS |
| Phasing software | CCP4 |
| Refinement software | SHELXL-97 |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 27.450 |
| High resolution limit [Å] | 0.990 |
| Rmerge | 0.060 |
| Number of reflections | 17058 |
| Completeness [%] | 94.7 |
| Redundancy | 3.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 7 | 5MG/ML POLYPEPTIDE IN 0.1M TRIS-HCL BUFFER, 0.001M ZN(OAC)2, pH 7.00 |
