2BF1
Structure of an unliganded and fully-glycosylated SIV gp120 envelope glycoprotein
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | CHESS BEAMLINE F1 |
| Synchrotron site | CHESS |
| Beamline | F1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2002-10-07 |
| Detector | ADSC CCD |
| Spacegroup name | P 43 21 2 |
| Unit cell lengths | 108.048, 108.048, 117.701 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 26.000 - 4.000 |
| R-factor | 0.385 |
| Rwork | 0.385 |
| R-free | 0.38800 |
| Structure solution method | MIRAS |
| RMSD bond length | 0.014 |
| RMSD bond angle | 2.067 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | SOLVE |
| Refinement software | REFMAC (5.2.0003) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 26.000 | 4.090 |
| High resolution limit [Å] | 4.000 | 4.000 |
| Rmerge | 0.080 | 0.820 |
| Number of reflections | 100402 | |
| <I/σ(I)> | 12 | 1.3 |
| Completeness [%] | 98.0 | 94.3 |
| Redundancy | 4.5 | 4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 5 | 293 | PROTEIN WAS CRYSTALLIZED FROM 15% PEG 6000, 100 MM SODIUM CITRATE, PH 5.0 AND 8% PEG 400 AT 20 DEGREES C. |
