2BER
Y370G Active Site Mutant of the Sialidase from Micromonospora viridifaciens in complex with beta-Neu5Ac (sialic acid).
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID14-3 |
| Synchrotron site | ESRF |
| Beamline | ID14-3 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2004-04-23 |
| Detector | MARRESEARCH |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 55.440, 49.535, 107.041 |
| Unit cell angles | 90.00, 101.74, 90.00 |
Refinement procedure
| Resolution | 105.410 - 1.800 |
| R-factor | 0.143 |
| Rwork | 0.140 |
| R-free | 0.19900 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1w8o |
| RMSD bond length | 0.020 |
| RMSD bond angle | 1.779 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | AMoRE |
| Refinement software | REFMAC (5.2.0005) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 34.940 | 1.900 |
| High resolution limit [Å] | 1.790 | 1.800 |
| Rmerge | 0.090 | 0.220 |
| Number of reflections | 53080 | |
| <I/σ(I)> | 11.1 | 3.1 |
| Completeness [%] | 87.3 | 87.3 |
| Redundancy | 2.7 | 2.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 5 | 16 % PEG 3350, 0.2 M SODIUM FLUORIDE., pH 5.00 |
