2BDR
Crystal Structure of the Putative Ureidoglycolate hydrolase PP4288 from Pseudomonas putida, Northeast Structural Genomics Target PpR49
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X4A |
| Synchrotron site | NSLS |
| Beamline | X4A |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2005-10-11 |
| Detector | ADSC QUANTUM 4 |
| Wavelength(s) | 0.979 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 83.027, 55.268, 81.843 |
| Unit cell angles | 90.00, 93.98, 90.00 |
Refinement procedure
| Resolution | 31.130 - 1.600 |
| R-factor | 0.198 |
| Rwork | 0.198 |
| R-free | 0.21800 |
| Structure solution method | SAD |
| RMSD bond length | 0.005 |
| RMSD bond angle | 1.300 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | SnB |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.660 |
| High resolution limit [Å] | 1.600 | 1.600 |
| Rmerge | 0.112 | 0.355 |
| Number of reflections | 92866 | |
| <I/σ(I)> | 14.41 | 0.328 |
| Completeness [%] | 97.2 | 96.1 |
| Redundancy | 9.3 | 9.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7 | 293 | 100mM MOPS, 40% PEG400, 100mM NaNO3, 5mM DTT, pH 7, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
