2BDQ

Crystal Structure of the Putative Copper Homeostasis Protein CutC from Streptococcus agalactiae, Northeast Strucural Genomics Target SaR15.

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	NSLS BEAMLINE X4A
Synchrotron site	NSLS
Beamline	X4A
Temperature [K]	100
Detector technology	CCD
Collection date	2005-10-15
Detector	ADSC QUANTUM 4
Wavelength(s)	0.979
Spacegroup name	P 21 21 21
Unit cell lengths	48.458, 71.542, 128.187
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	29.250 - 2.300
R-factor	0.221
Rwork	0.221
R-free	0.27100
Structure solution method	SAD
RMSD bond length	0.006
RMSD bond angle	1.200
Data reduction software	HKL-2000
Data scaling software	SCALEPACK
Phasing software	SnB
Refinement software	CNS (1.1)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.380
High resolution limit [Å]	2.300	2.300
Rmerge	0.126	0.368
Number of reflections	36017
<I/σ(I)>	19.71	9.61
Completeness [%]	99.9	100
Redundancy	13.5	13.5

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.5	293	10 mM Tris, 18% PEG3350, 200 mM NaSCN, 5 mM DTT, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 293K