2BDD

Crystal Structure of Holo-ACP-synthase from Plasmodium yoelii

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU FR-E
Temperature [K]	100
Detector technology	IMAGE PLATE
Collection date	2005-10-06
Detector	RIGAKU RAXIS IV
Wavelength(s)	1.54
Spacegroup name	P 21 3
Unit cell lengths	86.771, 86.771, 86.771
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	28.900 - 2.280
R-factor	0.2027
Rwork	0.200
R-free	0.25146
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1f80
RMSD bond length	0.024
RMSD bond angle	1.792
Data scaling software	HKL-2000
Phasing software	PHASER (V. 1.3.1)
Refinement software	REFMAC (5.2.0005)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	28.900	2.320
High resolution limit [Å]	2.280	2.280
Rmerge	0.099	0.936
Number of reflections	10191
<I/σ(I)>	6.9	2.8
Completeness [%]	100.0	100
Redundancy	14.2	14.3

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6.5	297	16% PEG8000, 0.2M Mg acetate, 0.1M Na Cacodylate, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 297K