2BCZ
Crystal Structure of a minimal, mutant all-RNA hairpin ribozyme (U39C, G8I, 2'deoxy A-1)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | CHESS BEAMLINE A1 |
| Synchrotron site | CHESS |
| Beamline | A1 |
| Temperature [K] | 83 |
| Detector technology | CCD |
| Collection date | 2005-05-05 |
| Detector | ADSC QUANTUM 210 |
| Wavelength(s) | 0.9764 |
| Spacegroup name | P 61 2 2 |
| Unit cell lengths | 94.490, 94.490, 127.580 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 44.300 - 2.400 |
| R-factor | 0.24 |
| Rwork | 0.240 |
| R-free | 0.27000 |
| Structure solution method | FOURIER SYNTHESIS |
| Starting model (for MR) | 1ZFR |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.500 |
| Data reduction software | CrystalClear ((MSC/RIGAKU)) |
| Data scaling software | CrystalClear ((MSC/RIGAKU)) |
| Phasing software | CNS (1.1) |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 44.300 | 2.480 |
| High resolution limit [Å] | 2.400 | 2.400 |
| Rmerge | 0.043 | 0.430 |
| Number of reflections | 13689 | |
| <I/σ(I)> | 19.1 | 4.3 |
| Completeness [%] | 99.2 | 98.7 |
| Redundancy | 6.25 | 5.91 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.2 | 293 | PEG550 MME, lithium sulfate, spermidine, cobalt hexamine, , pH 7.2, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
Crystallization Reagents
| ID | crystal ID | solution ID | reagent name | concentration | details |
| 1 | 1 | 1 | PEG550 MME | ||
| 2 | 1 | 1 | lithium sulfate | ||
| 3 | 1 | 1 | spermidine | ||
| 4 | 1 | 1 | cobalt hexamine | ||
| 5 | 1 | 1 | H2O | ||
| 6 | 1 | 2 | PEG550 MME | ||
| 7 | 1 | 2 | lithium sulfate | ||
| 8 | 1 | 2 | cobalt hexamine |
