2BCX
Crystal structure of calmodulin in complex with a ryanodine receptor peptide
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2004-12-03 |
| Detector | RIGAKU RAXIS IV |
| Wavelength(s) | 1.5418 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 38.100, 44.100, 90.300 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 9.990 - 2.000 |
| R-factor | 0.214 |
| Rwork | 0.213 |
| R-free | 0.24900 |
| Structure solution method | SAD |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.200 |
| Data scaling software | d*TREK (9.1SSI) |
| Phasing software | SHARP |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 39.660 | 2.070 |
| High resolution limit [Å] | 2.000 | 2.000 |
| Rmerge | 0.038 | 0.129 |
| Number of reflections | 10802 | |
| <I/σ(I)> | 23.2 | 7.8 |
| Completeness [%] | 99.9 | 99.9 |
| Redundancy | 3.73 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 4.7 | 293 | 23% PEG 4000, 0.1 M sodium acetate, 5 mM DTT, pH 4.7, VAPOR DIFFUSION, SITTING DROP, temperature 293K |
