2BCC
STIGMATELLIN-BOUND CYTOCHROME BC1 COMPLEX FROM CHICKEN
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRL BEAMLINE BL7-1 |
| Synchrotron site | SSRL |
| Beamline | BL7-1 |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 1997-10-04 |
| Detector | MARRESEARCH |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 173.460, 182.450, 241.330 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 12.000 - 3.500 |
| R-factor | 0.284 |
| Rwork | 0.284 |
| R-free | 0.31700 |
| Structure solution method | MOLECULAR REPLACEMENT USING NATIVE STRUCTURE SOLVED BY THE SAME AUTHOR |
| Starting model (for MR) | PDB 1BCC |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.900 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | CCP4 ((ALMN) |
| Refinement software | CNS (0.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 10000.000 | 3.560 |
| High resolution limit [Å] | 3.000 | 3.380 |
| Rmerge | 0.131 | 0.459 |
| Number of reflections | 117928 | |
| <I/σ(I)> | 6 | 1.6 |
| Completeness [%] | 77.1 | 75 |
| Redundancy | 2.9 | 2.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 6.7 | 20MM KMES PH6.7, 75MM NACL, 10% GLYCEROL, AND 6% PEG4000, INHIBITOR WAS ADDED FROM ETHANOLIC SOLUTION |
