2BC5
Crystal structure of E. coli cytochrome b562 with engineered c-type heme linkages
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU RU200 |
| Temperature [K] | 100 |
| Detector technology | AREA DETECTOR |
| Collection date | 2004-11-01 |
| Detector | SDMS |
| Wavelength(s) | 1.5418 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 63.861, 68.158, 90.440 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 24.400 - 2.250 |
| Rwork | 0.239 |
| R-free | 0.27700 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 256b |
| RMSD bond length | 0.011 |
| RMSD bond angle | 19.000 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | MOLREP |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 24.400 | 2.300 |
| High resolution limit [Å] | 2.250 | 2.250 |
| Rmerge | 0.079 | 0.296 |
| Number of reflections | 19081 | |
| <I/σ(I)> | 13.3 | 3.2 |
| Completeness [%] | 98.7 | 95.5 |
| Redundancy | 3.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 5 | 298 | 70% ammonium sulfate, pH 5, VAPOR DIFFUSION, SITTING DROP, temperature 298K |
