2BBE
Crystal structure of protein SO0527 from Shewanella oneidensis
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-ID |
| Synchrotron site | APS |
| Beamline | 19-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2005-08-23 |
| Detector | ADSC QUANTUM 4 |
| Wavelength(s) | 0.97956 |
| Spacegroup name | I 41 2 2 |
| Unit cell lengths | 94.191, 94.191, 97.147 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 50.000 - 1.970 |
| R-factor | 0.17347 |
| Rwork | 0.172 |
| R-free | 0.19536 |
| Structure solution method | SAD |
| RMSD bond length | 0.015 |
| RMSD bond angle | 1.314 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | HKL-3000 |
| Refinement software | REFMAC (5.2.0005) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.030 |
| High resolution limit [Å] | 1.960 | 1.960 |
| Rmerge | 0.073 | 0.241 |
| Number of reflections | 15765 | |
| <I/σ(I)> | 36.4 | 2.61 |
| Completeness [%] | 98.1 | 80.6 |
| Redundancy | 7.4 | 6.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 5.5 | 291 | 1.0 M Ammonium sulfate 0.1 M Bis-Tris 1 % PEG 3350, pH 5.5, VAPOR DIFFUSION, SITTING DROP, temperature 291K |
