2BBD
Crystal Structure of the STIV MCP
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALS BEAMLINE 12.3.1 |
| Synchrotron site | ALS |
| Beamline | 12.3.1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2004-09-18 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.97949 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 241.600, 82.890, 114.800 |
| Unit cell angles | 90.00, 116.00, 90.00 |
Refinement procedure
| Resolution | 29.760 - 2.040 |
| R-factor | 0.207 |
| Rwork | 0.207 |
| R-free | 0.24600 |
| Structure solution method | SAD |
| RMSD bond length | 0.006 |
| RMSD bond angle | 1.400 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | SnB |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 2.170 |
| High resolution limit [Å] | 2.040 | 2.040 |
| Number of reflections | 114006 | |
| Completeness [%] | 83.1 | 50 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 4.5 | 298.5 | 16% PEG 3350 10% MPD 0.2M Ammonium Citrate (pH 4.5) 5% (v/v) glycerol 2mM DTT 20mM N,N-Dimethyldecylamine-B-oxide 0.1M Bicine (pH 9.0), VAPOR DIFFUSION, HANGING DROP, temperature 298.5K |
