2BB3
Crystal Structure of Cobalamin Biosynthesis Precorrin-6Y Methylase (cbiE) from Archaeoglobus fulgidus
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 19-ID |
Synchrotron site | APS |
Beamline | 19-ID |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2004-10-28 |
Detector | ADSC QUANTUM 315 |
Wavelength(s) | 0.97932 |
Spacegroup name | C 2 2 21 |
Unit cell lengths | 88.191, 88.418, 115.932 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 29.100 - 2.270 |
R-factor | 0.211 |
Rwork | 0.211 |
R-free | 0.27400 |
Structure solution method | SAD |
RMSD bond length | 0.011 |
RMSD bond angle | 1.700 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | SHARP |
Refinement software | CNS (1.1) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 44.210 | 2.280 |
High resolution limit [Å] | 2.200 | 2.200 |
Rmerge | 0.068 | 0.808 |
Number of reflections | 22771 | |
<I/σ(I)> | 13.5 | 2.56 |
Completeness [%] | 97.3 | 82.2 |
Redundancy | 11 | 5.9 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 4.6 | 295 | 0.1M Sodium Acetate, 11% PEG10K, 2% Iso-Propanol,10mM SAH, pH 4.6, VAPOR DIFFUSION, HANGING DROP, temperature 295K |